Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene

Summary of the Study

• This study aimed to detect Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks.
• The study used a nested PCR technique to detect the presence of E. vermicularis in the FFPE blocks.
• The study also used a PCR-RFLP technique to compare the genetic variations of E. vermicularis based on the mitochondrial DNA (cox1) gene.
• The results of the study showed that E. vermicularis was detected in the FFPE blocks.
• The study also showed that there were significant differences in the genetic variations of E. vermicularis based on the mitochondrial DNA (cox1) gene.

Detailed Overview of the Study

This study aimed to detect Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks. The study used a nested PCR technique to detect the presence of E. vermicularis in the FFPE blocks. The study also used a PCR-RFLP technique to compare the genetic variations of E. vermicularis based on the mitochondrial DNA (cox1) gene.

The results of the study showed that E. vermicularis was detected in the FFPE blocks. The study also showed that there were significant differences in the genetic variations of E. vermicularis based on the mitochondrial DNA (cox1) gene. The study concluded that the nested PCR technique is a reliable method for detecting E. vermicularis in FFPE blocks and that the PCR-RFLP technique is a useful tool for comparing the genetic variations of E. vermicularis based on the mitochondrial DNA (cox1) gene.

The study also highlighted the potential of using archived FFPE blocks to study the genetic variations of E. vermicularis. This is important as it can provide valuable information about the epidemiology of E. vermicularis and its potential to spread in different geographical areas. Furthermore, the study showed that the nested PCR technique is a reliable method for detecting E. vermicularis in FFPE blocks and that the PCR-RFLP technique is a useful tool for comparing the genetic variations of E. vermicularis based on the mitochondrial DNA (cox1) gene.

Overall, this study provides valuable information about the potential of using archived FFPE blocks to study the genetic variations of E. vermicularis. The study also highlights the importance of using the nested PCR technique and the PCR-RFLP technique to detect and compare the genetic variations of E. vermicularis. This information can be used to better understand the epidemiology of E. vermicularis and its potential to spread in different geographical areas.

source of this article

published: 2023;
Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It’s potential to compare genetic variations based on mitochondrial DNA (cox1) gene

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